Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA expression of CTR1 (A), ATP7A (B), ATOX1 (C), ASCT2 (D) and PepT1 (E) genes in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA Expression of CTR1 (A), ATP7A (B), ATP7B (C), ATOX1 (D), ASCT2 (E) and PepT1 (F) genes in liver. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the protein expression for CTR1(A), and PepT1(B) in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal:

Article Title:

doi:

Figure Lengend Snippet: Scheme of the heme biosynthesis pathway and membrane transporters. ALA is synthesized from glycine and succinyl CoA endogenously or exogenously absorbed through PEPT-1. Protoporphyrin IX synthesized from ALA via enzymatic reactions, then catabolized to heme by FECH or eliminated through ABCG2. ABC, ATP-binding cassette; ALA, 5-aminolevulinic acid; ALAS, 5-aminolevulinic acid synthase; CPOX, coproporphyrinogen oxidase; FECH, ferrochelatase; PBGD, porphobilinogen deaminase; PBGS, porphobilinogen synthase; PEPT-1, oligopeptide transporter-1; PPOX, protoporphyrinogen IX oxidase; UROD, uroporphyrinogen III decarboxylase; UROS, uroporphyrinogen III synthase.

Article Snippet: The rabbit polyclonal anti-PEPT-1 antibody (Abcam PLC., Tokyo, Japan), rabbit monoclonal anti-β-actin (D6A8) antibody (CST Japan Co., Ltd, Tokyo, Japan), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG H&L (Abcam PLC., Tokyo, Japan) were used.

Techniques: Synthesized, Binding Assay

Journal:

Article Title:

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Figure Lengend Snippet: mRNA expression levels of membrane transporters. The relative mRNA expression levels of (A) PEPT-1, (B) ABCG2, and (C) ABCB6 assessed using quantitative reverse transcription-polymerase chain reaction in hPDECs and pancreatic cancer cells. The relative levels compared to hPDECs are shown with a logarithmic scale. Bars indicate average ± SEM. *P < 0.01 compared to the expression levels in hPDECs analyzed by one-way ANOVA followed by post hoc Dunnet test. ABC, the ATP-binding cassette; hPDEC, human pancreatic duct epithelial cell; PEPT-1, oligopeptide transporter-1.

Article Snippet: The rabbit polyclonal anti-PEPT-1 antibody (Abcam PLC., Tokyo, Japan), rabbit monoclonal anti-β-actin (D6A8) antibody (CST Japan Co., Ltd, Tokyo, Japan), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG H&L (Abcam PLC., Tokyo, Japan) were used.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay

Journal:

Article Title:

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Figure Lengend Snippet: Protein expression levels of PEPT-1 in hPDECs, MIA PaCa-2, and AsPC-1 cells. (A) Representative image of western blot for PEPT-1 and β-actin in hPDECs, MIA PaCa-2, and AsPC-1 cells. (B) Quantitative analyses of the western blots for PEPT-1 protein from 3 repetitive experiments. The signal intensity was normalized to the β-actin protein level and expressed as relative expression levels in hPDECs. Bars indicate average ± SEM. *P < 0.05 compared to hPDECs. One-way ANOVA followed by post hoc Tukey test. hPDEC, human pancreatic duct epithelial cell; PEPT-1, oligopeptide transporter-1.

Article Snippet: The rabbit polyclonal anti-PEPT-1 antibody (Abcam PLC., Tokyo, Japan), rabbit monoclonal anti-β-actin (D6A8) antibody (CST Japan Co., Ltd, Tokyo, Japan), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG H&L (Abcam PLC., Tokyo, Japan) were used.

Techniques: Expressing, Western Blot

Journal:

Article Title:

doi:

Figure Lengend Snippet: PpIX accumulation in hPDECs, MIA PaCa-2, and AsPC-1 cells incubated with ALA and the PEPT-1 inhibitor P-(aminomethyl) benzoic acid. (A) Representative images of fluorescence in hPDECs, MIA PaCa-2, and AsPC-1 cells after ALA administration with or without AMBA at a final concentration of 30 mM. Bar = 200 μm. (B) Quantitative analyses for fluorescence intensities in hPDECs, MIA PaCa-2, and AsPC-1 cells, expresses as the relative intensity to hPDECs without AMBA. Bars in dicate average ± SEM. *P < 0.05, ***P < 0.001 (Mann-Whitney U test in each cell line). ALA, 5-aminolevulinic acid; hPDEC, human pancreatic duct epithelial cell.

Article Snippet: The rabbit polyclonal anti-PEPT-1 antibody (Abcam PLC., Tokyo, Japan), rabbit monoclonal anti-β-actin (D6A8) antibody (CST Japan Co., Ltd, Tokyo, Japan), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG H&L (Abcam PLC., Tokyo, Japan) were used.

Techniques: Incubation, Fluorescence, Concentration Assay, MANN-WHITNEY

Journal:

Article Title:

doi:

Figure Lengend Snippet: PpIX accumulation in pancreatic cancer cell lines after administration of ALA. (A) Representative images of fluorescence in pancreatic cancer cell lines with relatively high expression of PEPT-1. Images were captured 1 h after the administration of ALA in SUIT-2, AsPC-1, KP-3L, and Capan-1 cells. (B) Representative images of fluorescence in cell lines with relatively low expression of PEPT-1. Images were captured 1 h after the administration of ALA in TYPK-1, MIA PaCa-2, PANC-1, KP-4, and BxPC-3 cells. Bar = 100 μm.

Article Snippet: The rabbit polyclonal anti-PEPT-1 antibody (Abcam PLC., Tokyo, Japan), rabbit monoclonal anti-β-actin (D6A8) antibody (CST Japan Co., Ltd, Tokyo, Japan), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG H&L (Abcam PLC., Tokyo, Japan) were used.

Techniques: Fluorescence, Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Primer sequences for real-time PCR amplification.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Sequencing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, ( n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Antioxidants

Article Title: The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid

doi: 10.3390/antiox10101531

Figure Lengend Snippet: Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and SLC15A1 ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.

Article Snippet: Antirabbit SLC22A16 (cat. no. LS-C176922) (LSBio, Seattle, WA, USA), ABCG2 (cat. no. 42078) (Cell Signaling Technology Japan K.K., Tokyo, Japan), or SLC15A1 (cat. no. bs-10588R) (Bioss, Boston, MA, USA) antibodies (1:1000) were added to the Can Get Signal ® Immunoreaction Enhancer Solution 1 (Toyobo Co. Ltd., Osaka, Japan).

Techniques: Western Blot, Expressing